An integrated bioprocess for fermentative production of protopanaxadiol by recycling ethanol waste during down-stream extraction process. (10th October 2019)

• Record Type: Journal Article
• Authors: Zhao, Fanglong; Zhang, Chuanbo; Nan, Weihua; Lu, Wenyu
• Is Part Of: Journal of cleaner production. Volume 234(2019)
• Page Start: 1
• Page End: 8
• Publication Date: 2019-10-10
• Subjects: Integrated bioprocess -- Protopanaxadiol -- Fermentation -- Organic solvent recovery -- Saccharomyces cerevisiae; Factory and trade waste -- Management -- Periodicals; Manufactures -- Environmental aspects -- Periodicals; Déchets industriels -- Gestion -- Périodiques; Usines -- Aspect de l'environnement -- Périodiques; 628.5
• DOI: 10.1016/j.jclepro.2019.06.190
• Languages: English
• ISSNs: 0959-6526

Abstract

Abstract: Solvent recovery and reutilization in bioprocess have great potential to reduce production cost and environmental harm. However, solvent recovery process is often energy-intensive and costly. In this study, we developed an integrated bioprocess for fermentative production of protopanaxadiol (PPD) from ethanol waste recycled in down-stream extraction process, achieving solvent recovery and reutilization in a cost-effective manner. In this integrated process, PPD isolation and purification were achieved by foam separation and resin chromatography, respectively; ethanol solution used as PPD extractant and chromatography eluent was recycled to be reused directly as yeast carbon source for PPD biosynthesis. Notably, in 3 batches of 5 L-fermentation productions, the recycled ethanol could compensate 81.3% of the ethanol used in fermentation. Since the lost PPD during chromatography elution was returned into the next-batch fermentation together with the recycled ethanol, the overall yield of 3-batch production (85.78%) was higher than the yield of single batch production (∼75%). This study demonstrates a promising integrated bioprocess for triterpene compounds production from ethanol waste. Highlights: An integrated bioprocesse was designed to recycle organic solvent and provide cost saving for protopanoxadiol (PPD) production. This integrated bioprocesse also simplified the PPD extraction process. The recycled ethanol can be reutilized as fermentation carbon source completely. The yield of the integrated bioprocesses was about 10% higher than single batch yield.