Tropomyosins in mosquito and house dust mite cross‐react at the humoral and cellular level. Issue 10 (14th August 2018)

• Record Type: Journal Article
• Authors: Cantillo, Jose F.; Puerta, Leonardo; Fernandez‐Caldas, Enrique; Subiza, Jose L.; Soria, Irene; Wöhrl, Stefan; Ebner, Christof; Keller, Walter; Resch‐Marat, Yvonne; Vrtala, Susanne; Bohle, Barbara
• Is Part Of: Clinical & experimental allergy. Volume 48:Issue 10(2018)
• Page Start: 1354
• Page End: 1363
• Publication Date: 2018-08-14
• Subjects: cross‐reactivity -- house dust mites -- mosquito -- T‐cell epitopes -- tropomyosin; Allergy -- Periodicals; Immunology -- Periodicals; 616.97
• DOI: 10.1111/cea.13229
• Languages: English
• ISSNs: 0954-7894

Abstract

Summary: Background: Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross‐reactive tropomyosins. However, the functional and clinical relevance of their cross‐reactivity is still debated. Objective: To analyse the humoral and cellular cross‐reactivity of recombinant Aed a 10.01, Aed a 10.02 and Der p 10. Methods: Sera from 15 Austrian house dust mite‐allergic, Der p 10‐sensitized individuals were tested for IgE reactivity to recombinant tropomyosins in ELISA, inhibition ELISA and basophil activation tests. BALB/c mice were immunized with Aed a 10.01 or Aed a 10.02, and their sera were assessed for reactivity to all tropomyosins. Splenocytes were stimulated with all tropomyosins and synthetic peptides representing the amino acid sequence of Aed a 10.01. Results: IgE antibodies of Der p 10‐sensitized patients cross‐reacted with both tropomyosins from A. aegypti . Aed a 10.01 was a more potent inhibitor of IgE binding to Der p 10 and a stronger activator of basophils sensitized with Der p 10‐specific IgE than Aed a 10.02. Murine antibodies raised against Aed a 10.01 and Aed a 10.02 cross‐reacted with Der p 10. Aed a 10.01‐specific antibody showed stronger cross‐reactivity with Der p 10 than Aed a 10.02‐specific antibody. Splenocytes from both groups of mice proliferated similarly to all tropomyosins. Five cross‐reactive T cell‐activating regions were identified. Conclusion and Clinical relevance: Tropomyosins from D. pteronyssinus and A. aegypti show humoral and cellular cross‐reactivity, involving 5 potential T cell‐activating regions. The more pronounced cross‐reactivity of Aed a 10.01 and Der p 10 matched the higher sequence similarity of both proteins.