Effect of static scatterers in laser speckle contrast imaging: an experimental study on correlation and contrast. (29th December 2017)

• Record Type: Journal Article
• Authors: Vaz, Pedro G; Humeau-Heurtier, Anne; Figueiras, Edite; Correia, Carlos; Cardoso, João
• Is Part Of: Physics in medicine & biology. Volume 63:Number 1(2018:Jan.)
• Publication Date: 2017-12-29
• Subjects: laser speckle contrast imaging -- speckle correlation -- spatial contrast -- static scatterers; Biophysics -- Periodicals; Medical physics -- Periodicals; 610.153
• DOI: 10.1088/1361-6560/aa9f3a
• Languages: English
• ISSNs: 0031-9155

Abstract

Abstract: Laser speckle contrast imaging (LSCI) is a non-invasive microvascular blood flow assessment technique with good temporal and spatial resolution. Most LSCI systems, including commercial devices, can perform only qualitative blood flow evaluation, which is a major limitation of this technique. There are several factors that prevent the utilization of LSCI as a quantitative technique. Among these factors, we can highlight the effect of static scatterers. The goal of this work was to study the influence of differences in static and dynamic scatterer concentration on laser speckle correlation and contrast. In order to achieve this, a laser speckle prototype was developed and tested using an optical phantom with various concentrations of static and dynamic scatterers. It was found that the laser speckle correlation could be used to estimate the relative concentration of static/dynamic scatterers within a sample. Moreover, the speckle correlation proved to be independent of the dynamic scatterer velocity, which is a fundamental characteristic to be used in contrast correction.