Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method. Issue 2 (2003)
- Record Type:
- Journal Article
- Title:
- Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method. Issue 2 (2003)
- Main Title:
- Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method
- Authors:
- Kamagata Kamagata, Kiyoto Kiyoto
Kuwajima Kuwajima, Kunihiro Kunihiro - Abstract:
- Abstract : The folding mechanism of proline‒free staphylococcal nuclease (SNase (pro − )) (P11A, P31A, P42A, P47T, P56A, P117G) was investigated using the double‒jump stopped‒flow method (interrupted refolding). This method has enabled us to specifically monitor the amount of the native molecules during the refolding. The results indicate that the middle and slow phases observed in the refolding kinetics represent the formation of the native state (IM →N, IS →N) and that the folding mechanism of SNase (pro − ) is not represented by a single sequential pathway, but at least two parallel pathways are required for interpreting the results.
- Is Part Of:
- Spectroscopy. Volume 17:Issue 2/3(2003)
- Journal:
- Spectroscopy
- Issue:
- Volume 17:Issue 2/3(2003)
- Issue Display:
- Volume 17, Issue 2/3 (2003)
- Year:
- 2003
- Volume:
- 17
- Issue:
- 2/3
- Issue Sort Value:
- 2003-0017-NaN-0000
- Page Start:
- 203
- Page End:
- 212
- Publication Date:
- 2003
- DOI:
- 10.1155/2003/252581 ↗
- Languages:
- English
- ISSNs:
- 0712-4813
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 11274.xml