Protein characterization using Liquid Chromatography Desorption Ionization on Silicon Mass Spectrometry (LC-DIOS-MS). Issue 4 (2003)

• Record Type: Journal Article
• Authors: Prenni Prenni, Jessica E. Jessica E.; Shen Shen, Zhouxin Zhouxin; Trauger Trauger, Sunia Sunia; Chen Chen, Wei Wei; Siuzdak Siuzdak, Gary Gary
• Is Part Of: Spectroscopy. Volume 17:Issue 4(2003)
• Page Start: 693
• Page End: 698
• Publication Date: 2003
• DOI: 10.1155/2003/172838
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : This paper presents a novel combination of of-line liquid chromatography (LC) separation with desorption ionization on silicon mass spectrometry (DIOS-MS) as an alternative to the traditional on-line coupling of LC and electrospray ionization (ESI). In this work, electrospray deposition is used to generate a spatially preserved linear track of the separated sample on a specially designed DIOS chip. The total sequence coverage analysis of two model protein systems was evaluated by both LC-DIOS-MS and nanoLC ESI tandem MS (MS/MS). LC-DIOS-MS yielded improved sequence coverage for both of the model systems (between 99.5 and 100%) compared with traditional LC-ESI-MS/MS analysis (between 82 and 87.6%). In addition to improved sequence coverage determination, LC-DIOS-MS also offers the potential for high-throughput protein characterization.