Protein Corona Liposomes Achieve Efficient Oral Insulin Delivery by Overcoming Mucus and Epithelial Barriers. Issue 12 (28th November 2018)

• Record Type: Journal Article
• Authors: Wang, Aohua; Yang, Tiantian; Fan, Weiwei; Yang, Yiwei; Zhu, Quanlei; Guo, Shiyan; Zhu, Chunliu; Yuan, Yongchun; Zhang, Tao; Gan, Yong
• Is Part Of: Advanced healthcare materials. Volume 8:Issue 12(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-11-28
• Subjects: insulin -- intestinal epithelium -- mucus layers -- oral delivery -- protein corona liposomes; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201801123
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Oral delivery of peptide/protein drugs has attracted worldwide attention due to its good patient compliance and convenience of administration. Orally administered nanocarriers always encounter the rigorous defenses of the gastrointestinal tract, which mainly consist of mucus and epithelium barriers. However, diametrically opposite surface properties of nanocarriers are required for good mucus penetration and high epithelial uptake. Here, bovine serum albumin (BSA) is adsorbed to cationic liposomes (CLs) to form protein corona liposomes (PcCLs). The aim of using PcCLs is to conquer the mucus and epithelium barriers, eventually improving the oral bioavailability of insulin. Investigations using in vitro and in vivo experiments show that the uptake amounts and transepithelial permeability of PcCLs are 3.24‐ and 7.91‐fold higher than that of free insulin, respectively. Further study of the behavior of PcCLs implies that BSA corona can be shed from PcCLs as they cross the mucus layer, which results in the exposure of CLs to improve the transepithelial transport. Intrajejunal administration of PcCLs in type I diabetic rats produces a remarkable hypoglycemic effect and increases the oral bioavailability up to 11.9%. All of these results imply that PcCLs may provide a new insight into the method for oral insulin delivery by overcoming the multiple barriers. Abstract : Protein corona are composed of cationic liposomes (CLs) as the nanocore and bovine serum albumin (BSA) as the protein corona. The PcCLs exhibit faster mucus penetration due to the neutral charge, and improve the cellular internalization as the exposed CLs after BSA degradation in the mucus layer, thus these PcCLs can enhance the oral bioavailability of insulin.