Protein crystal structure determination with the crystallophore, a nucleating and phasing agent. Issue 4 (28th June 2019)

• Record Type: Journal Article
• Authors: Engilberge, Sylvain; Wagner, Tristan; Santoni, Gianluca; Breyton, Cécile; Shima, Seigo; Franzetti, Bruno; Riobé, Francois; Maury, Olivier; Girard, Eric
• Is Part Of: Journal of applied crystallography. Volume 52:Issue 4(2019)
• Page Start: 722
• Page End: 731
• Publication Date: 2019-06-28
• Subjects: the crystallophore -- Tb‐Xo4 -- macromolecular crystallization -- de novo phasing -- anomalous‐scattering‐based methods -- protein crystallography -- serial crystallography; Crystallography -- Periodicals; 548.05
• DOI: 10.1107/S1600576719006381
• Languages: English
• ISSNs: 0021-8898

Abstract

Abstract : The unique nucleating and phasing capabilities of the crystallophore, Tb‐Xo4, are illustrated through challenging cases. Abstract : Obtaining crystals and solving the phase problem remain major hurdles encountered by bio‐crystallographers in their race to obtain new high‐quality structures. Both issues can be overcome by the crystallophore, Tb‐Xo4, a lanthanide‐based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb‐Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low‐resolution diffracting samples or crystals with twinning. Thanks to Tb‐Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.