Green Fluorescent Protein Nanovessel Serves as a Nucleolus Targeting Material and Molecule Carrier in Living Cells. Issue 7 (3rd June 2019)

• Record Type: Journal Article
• Authors: Li, Zhenhua; Li, Jia; Liu, Shi; Wang, Haojie; Xie, Zhigang; Wang, Youjun; Chen, Zhijun
• Is Part Of: Advanced biosystems. Volume 3:Issue 7(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-06-03
• Subjects: drug delivery -- green florescent protein nanovessels -- nucleolus imaging -- ThT fluorescence light up; Biological systems -- Periodicals; Biotechnology -- Periodicals; Bioengineering -- Periodicals; Biomedical engineering -- Periodicals; Biological Science Disciplines; Periodicals; Periodicals; 660.6
• DOI: 10.1002/adbi.201900047
• Languages: English
• ISSNs: 2366-7478

Abstract

Abstract: The nucleolus is responsible for RNA transcription, processing, and ribosome assembly, the dysfunction of which is associated with a number of diseases. In this report, a new member of fluorescent protein nanovessels (FPNs), constructed using thioflavin‐T (ThT) and bovine serum albumin (BSA) as building blocks, is described. As a popular amyloid specific dye, ThT is nonfluorescent by itself, while its fluorescence can be lighted up upon interacting with amyloid proteins. Herein, ThT is coassembled with the BSA scaffold at high temperature to form T(hT)‐FPNs. These green fluorescence emissive bio‐abiotic hybrid materials can serve as a novel probe for real‐time nucleolus imaging of living cells. Besides, T‐FPNs show potential in delivering insoluble and/or impenetrable drugs into living cells, suggesting another role as a molecule carrier. Abstract : Thioflavin‐T (ThT) is coassembled with bovine serum albumin to form T(hT)‐fluorescent protein nanovessels (FPNs). These green fluorescence emissive bio‐abiotic hybrid materials can serve as a novel probe for real‐time nucleolus imaging of living cells. Besides, T‐FPNs show potential in delivering insoluble and/or impenetrable drugs into living cells, suggesting another role as a molecule carrier.