Front Cover: Identification of mNeonGreen as a pH‐Dependent, Turn‐On Fluorescent Protein Sensor for Chloride (ChemBioChem 14/2019). (2nd July 2019)

• Record Type: Journal Article
• Authors: Tutol, Jasmine N.; Kam, Hiu C.; Dodani, Sheel C.
• Is Part Of: Chembiochem. Volume 20:Number 14(2019)
• Page Start: 1727
• Page End: 1727
• Publication Date: 2019-07-02
• Subjects: anions -- chloride binding pocket -- fluorescent protein sensor -- mNeonGreen -- protein engineering; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201900408
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : mNeonGreen: A bright idea for chloride : Chloride‐sensitive fluorescent proteins generated from natural and laboratory‐guided evolution have a characteristic tyrosine residue that interacts with the anion and π‐stacks with the chromophore. Through a structure‐guided approach, we have identified that the fluorescent protein mNeonGreen, based on lan YFP from the cephalochordate Branchiostoma lanceolatum, lacks this interaction but still binds chloride. Here, we show mNeonGreen displayed as the light bulb filament, lighting up in the presence of chloride to generate a pH‐dependent, turn‐on fluorescent response. This discovery sets the stage to further engineer mNeonGreen as a new tool for imaging cellular chloride dynamics. More information can be found in the communication by S. C. Dodani et al. onpage 1759 in Issue 14, 2019 (DOI:10.1002/cbic.201900147 ).