Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells. Issue 9 (2nd September 2019)

• Record Type: Journal Article
• Authors: Boutaoui, Nadia; Puranik, Sandeep; Zhang, Rong; Wang, Ting; Hui, Daniel H.; Brehm, John; Forno, Erick; Chen, Wei; Celedón, Juan C.
• Is Part Of: Epigenetics. Volume 14:Issue 9(2019)
• Page Start: 844
• Page End: 849
• Publication Date: 2019-09-02
• Subjects: Vitamin D -- ATAC-Seq -- RNA-Seq -- asthma; Epigenesis -- Periodicals; Epigenetica; 572.86505
• DOI: 10.1080/15592294.2019.1622993
• Languages: English
• ISSNs: 1559-2294

Abstract

ABSTRACT: Vitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic changes in human bronchial epithelial cells of asthmatic subjects, following treatment of these cells with calcitriol (vitamin D3) and Poly (I:C)(a viral analogue). This approach led to the identification of biologically plausible candidate genes for viral infections and asthma, such as DUSP10 and SLC44A1.