Coreopsis tinctoria flowers extract ameliorates D-galactose induced aging in mice via regulation of Sirt1-Nrf2 signaling pathway. (September 2019)

• Record Type: Journal Article
• Authors: Tian, Yong; Wen, Zhen; Lei, Lin; Li, Fuhua; Zhao, Jichun; Zhi, Qi; Li, Fuxiang; Yin, Ran; Ming, Jian
• Is Part Of: Journal of functional foods. Volume 60(2019)
• Publication Date: 2019-09
• Subjects: Coreopsis tinctoria flowers -- UPLC/Q-TOF-MS -- Antioxidant -- Anti-aging -- Sirt1-Nrf2 signaling pathway; ABAP 2, 2′-azobis (2-amidinopropane) di-hydrochloride -- CTE Coreopsis tinctoria flowers extract -- D-gal D-galactose -- DPPH 1, 1-diphenyl-2-picrylhydrazyl -- FOXOs Forkhead transcription factors -- GSH-Px glutathione peroxidase -- HO-1 heme oxygenase-1 -- MDA malondialdehyde -- NF-κB nuclear factor κB -- NQO1 NAD(P)H: quinone oxidoreductase -- Nrf2 nuclear factor-erythroid-2-related factor 2 -- p53 tumor suppressor protein 53 -- ROS reactive oxygen species -- Sirt1 Sirtuin type 1 -- Sirt3 Sirtuin type 3 -- SOD superoxide dismutase -- T-AOC total antioxidant capacity -- UPLC-Q-TOF-MS ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103464
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: A total of 12 active compounds were identified in CTE using UPLC/Q-TOF-MS. CTE showed superior antioxidant activities in vitro. CTE exhibited promising anti-aging effects against D -Gal induced aging in mice. The underlying mechanism might be related to up-regulation of Sirt1-Nrf2 signaling pathway by CTE. Abstract: The aim of this study was to investigate the protective effects and its underlying signaling pathways of Coreopsis tinctoria flowers extract (CTE) on D -galactose induced aging in mice. UPLC/Q-TOF-MS analysis of CTE identified 12 active compounds, including three phenolic acids and nine flavonoids. The extract showed superior antioxidant activities in vitro using DPPH, FRAP, and ORAC assay. Administration with CTE remarkably improved body weight, organ indexes of aging mice, and liver histological injury as well as decreased oxidative stress markers levels of aged mice. Further studies showed CTE treatment significantly enhanced Sirt1 expression and activated the Nrf2 signaling pathway by the increase of Nrf2 nuclear translocation, NQO-1 and HO-1 expression. Furthermore, CTE treatment resulted in the increase of Sirt3 and the suppression of p53. These results demonstrated that the promising anti-aging effects of Coreopsis tinctoria flowers extract might be related to up-regulation of Sirt1-Nrf2 signaling pathway.