Single Muscle Fibre Contractility Testing in Rats to Quantify Ischaemic Muscle Damage During Reperfusion Injury. (August 2019)

• Record Type: Journal Article
• Authors: Gok, Emre; Alghanem, Fares; Lim, Eunice; Sarver, Dylan; Eckhardt, Logan; Christensen, Shelby; Hoenerhoff, Mark; Mendias, Christopher; Ozer, Kagan
• Is Part Of: European journal of vascular and endovascular surgery. Volume 58:Number 2(2019)
• Page Start: 249
• Page End: 256
• Publication Date: 2019-08
• Subjects: Single muscle fibre contractility -- Ischemia–reperfusion injury -- Muscle; Blood-vessels -- Endoscopic surgery -- Periodicals; Blood-vessels -- Surgery -- Periodicals; Vascular Surgical Procedures -- Periodicals; Vascular Surgical Procedures -- methods -- Periodicals; Vaisseaux sanguins -- Chirurgie -- Périodiques; Vaisseaux sanguins -- Chirurgie endoscopique -- Périodiques; Blood-vessels -- Endoscopic surgery; Blood-vessels -- Surgery; Endoscopy; Electronic journals; Periodicals; Electronic journals; 617.413005
• DOI: 10.1016/j.ejvs.2019.01.009
• Languages: English
• ISSNs: 1078-5884

Abstract

Abstract : Objectives: In this study, the aim was to investigate the potential for single muscle fibre contractility (SMFC) testing to detect the extent of reperfusion injury following various reperfusion periods. The hypothesis was that force generated by muscle fibres will correlate inversely with the extent of reperfusion injury. Methods: Twenty-four Lewis rats were distributed among five groups. Group 1 served as normal muscle control. In all other groups, femoral artery flow was occluded for four hours. Muscle biopsies were obtained at 0 hour, six hours, day two, and day seven after reperfusion in Groups 2, 3, 4, and 5, respectively. Samples then underwent ultrastructural analysis (H&E stain) and SMFC testing. Results: The maximum isometric force (mN) generated on Days two and seven after reperfusion decreased from baseline by 21% (p < 0.05), and 53% ( p < .001), respectively. The specific force (kPa) followed a similar pattern with a 13% decrease at Day two ( p > 0.05) and 31% decrease at Day 7 ( p < .001). These results correlated inversely with the extent of quantitative injury on histology. Conclusions: The study demonstrated an inverse relationship between single muscle fibre contractility testing and neutrophil infiltration during the reperfusion phase. Further clinical studies are needed to evaluate its potential in providing prognostic information for patient outcomes.