Dexmedetomidine preconditioning attenuates global cerebral ischemic injury following asphyxial cardiac arrest. (3rd March 2016)

• Record Type: Journal Article
• Authors: Ding, Xu-Dong; Zheng, Ning-Ning; Cao, Yan-Yan; Zhao, Guang-Yi; Zhao, Ping
• Is Part Of: International journal of neuroscience. Volume 126:Number 3(2016:Mar.)
• Page Start: 249
• Page End: 256
• Publication Date: 2016-03-03
• Subjects: dexmedetomidine -- global cerebral ischemia -- asphyxial cardiac arrest -- HIF-1α -- VEGF; Nervous system -- Periodicals; 612.805
• DOI: 10.3109/00207454.2015.1005291
• Languages: English
• ISSNs: 0020-7454

Abstract

Abstract : Background/Aims : To investigate the protection effect of dexmedetomidine preconditioning on global cerebral ischemic injury following asphyxial cardiac arrest (CA) in rats. Methods : Seventy-two rats were randomly assigned into three groups, sham group (no asphyxia), control group (asphyxia only), and dexmedetomidine preconditioned group (asphyxia + dexmedetomidine). Dexmedetomidine was administered 5 minutes before an 8 min of asphyxia. Rats were resuscitated by a standardized method. Blood O2 and CO2 partial pressures were, pH, base excess (BE), and blood glucose concentration measured before asphyxial CA and 1 h after resuscitation. Neurological deficit score (NDS) was measured at 12, 24, 48, and 72 h after CA. Histopathologic changes in the hippocampal region were observed by H&E staining and histopathologic damage score. Ultrastructural morphology was observed by transmission electron microscopy. HIF-1 and VEGF expression were measured by immunostaining of serial sections obtained from brain tissue. Results : Asphyxial CA -induced global cerebral ischemic decreased PaO2, pH, BE and increased PaCO2, blood glucose. Dexmedetomidine preconditioning improved neurologic outcome, which was associated with reduction in histopathologic injury measured by H&E staining, the histopathologic damage score and electron microscopy. Dexmedetomidine preconditioning also elevated HIF-1α and VEGF expression after global cerebral ischemia following asphyxial CA. Conclusion : Dexmedetomidine preconditioning protected against cerebral ischemic injury and was associated with upregulation of HIF-1α and VEGF expression.