Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Truong, Dieu-Hien; Nguyen, Hoang Chinh; Bauwens, Julien; Mazzucchelli, Gabriel; Lognay, Georges; Francis, Frédéric
• Is Part Of: Journal of plant interactions. Volume 13:Issue 1(2018)
• Page Start: 30
• Page End: 36
• Publication Date: 2018-01-01
• Subjects: Arabidopsis thaliana -- Plutella xylostella -- proteomic expression -- 2-DE -- MALDI-TOF MS -- LC-ESI-MS/MS; Plant ecophysiology -- Periodicals; Plants -- Periodicals; Toxins -- Periodicals; 581.7
• DOI: 10.1080/17429145.2017.1414320
• Languages: English
• ISSNs: 1742-9145

Abstract

ABSTRACT: The interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xylostella larval infestation at 27°C within 8 h. Approximately 450 protein spots were reproducibly detected on gels. Of these, comparing healthy and infested leaves, we identified 18 differentially expressed protein spots. Thirteen proteins were successfully identified by MALDI-TOF/MS and LC-ESI-MS/MS. Functional classification analysis indicated that the differentially identified proteins were associated with amino acid, carbohydrate, energy, lipid metabolism, and photosynthesis. In addition, their relative abundances were assessed according to larval pest feeding on Arabidopsis leaves. These data provide valuable new insights for further works in plant-biotic and environmental stress interaction.