New contributions for industrial n-butanol fermentation: An optimized Clostridium strain and the use of xylooligosaccharides as a fermentation additive. (December 2018)

• Record Type: Journal Article
• Authors: Grassi, M.C.B.; Carazzolle, M.F.; Nakagawa, B.T.; Ferrari, A.; Nagamatsu, S.; Santos, C.R.; Murakami, M.T.; Pirolla, R.A.S.; Pereira, G.A.G.
• Is Part Of: Biomass and bioenergy. Volume 119(2018)
• Page Start: 304
• Page End: 313
• Publication Date: 2018-12
• Subjects: ABE fermentation -- n-Butanol -- Cellulose pulp -- Lignocellulosic biomass -- Xylooligosaccharides; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2018.09.012
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: n-Butanol is a chemical widely used in industry, with an approximate annual output of 4 million tons and a market valued at USD 8.6 billion. It was first produced by ABE fermentation (acetone - n-butanol - ethanol) at the beginning of the twentieth century using molasses as a substrate, an expensive raw material that made the process economically unfeasible. In this work, we developed a second-generation fermentation process using cellulosic sugar as the substrate. A strain of Clostridium saccharoperbutylacetonicum has been evolved to n-butanol resistance, and we have found that the addition of xylooligosaccharides to the culture medium leads to a significant increase in the n-butanol yield and productivity. As a result, an n-butanol titer of 12.5 g/L and a productivity of 0.43 g/L.h were obtained, a respective gain of 17% and 58% relative to the initial process. These advances indicate that second-generation technologies can make viable the production of renewable n-butanol. Highlights: C. saccharoperbutylacetonicum produces n-butanol from sugarcane straw hydrolysate. Adaptive evolution is an efficient tool to develop n-butanol tolerant strains. Cellulose pulp can be added in the fermentation to improve bacterial performance. Xylooligosaccharides can act as a prebiotic stimulating the bacterial metabolism.