Emergence of IncX4 plasmids encoding mcr-1 in a clinical isolate of Klebsiella pneumoniae in Japan. (October 2018)

• Record Type: Journal Article
• Authors: Tada, Tatsuya; Uechi, Kohei; Nakasone, Isamu; Nakamatsu, Masashi; Satou, Kazuhito; Hirano, Takashi; Kirikae, Teruo; Fujita, Jiro
• Is Part Of: International journal of infectious diseases. Volume 75(2018)
• Page Start: 98
• Page End: 100
• Publication Date: 2018-10
• Subjects: Colistin resistance -- MCR-1 producing Klebsiella pneumoniae -- IncX4 plasmid harboring mcr-1; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2018.08.011
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: A colistin-resistant sequence type 1296 Klebsiella pneumoniae isolate harboring mcr-1 was isolated in a hospital in Japan. Pulsed-field gel electrophoresis and Southern hybridization revealed that mcr-1 was located on a 33-kb plasmid. The complete genome of the mcr-1 harboring plasmid with the size of 33 kb was determined; the plasmid incompatibility was IncX4. This is the first report of a K. pneumoniae clinical isolate harboring mcr-1 in Japan. Abstract: The mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to colistin by transferring phosphoethanolamine to lipid A. A 33-kb IncX4 plasmid harboring mcr-1 was detected in a Klebsiella pneumoniae isolate and two Escherichia coli isolates, and a 66-kb IncI2 plasmid was detected in three E. coli isolates in hospitals in Okinawa, Japan.