In vitro and clinical data analysis of Osteopontin as a prognostic indicator in colorectal cancer. Issue 9 (30th May 2018)

• Record Type: Journal Article
• Authors: Wei, Ran; Wong, Janet Pik Ching; Lyu, Peng; Xi, Xinping; Tong, Olivia; Zhang, Shu‐Dong; Yuen, Hiu Fung; Shirasawa, Senji; Kwok, Hang Fai
• Is Part Of: Journal of cellular and molecular medicine. Volume 22:Issue 9(2018)
• Page Start: 4097
• Page End: 4105
• Publication Date: 2018-05-30
• Subjects: colorectal cancer -- connectivity mapping -- Osteopontin -- survival; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.13686
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Osteopontin (OPN) has been shown to promote colorectal cancer (CRC) progression; however, the mechanism of OPN‐induced CRC progression is largely unknown. In this study, we found that OPN overexpression led to enhanced anchorage‐independent growth, cell migration and invasion in KRAS gene mutant cells but to a lesser extent in KRAS wild‐type cells. OPN overexpression also induced PI3K signalling, expression of Snail and Matrix metallopeptidase 9 (MMP9), and suppressed the expression of E‐cadherin in KRAS mutant cells. In human CRC specimens, a high‐level expression of OPN significantly predicted poorer survival in CRC patients and OPN expression was positively correlated with MMP9 expression, and negatively correlated with E‐cadherin expression. Furthermore, we have found that 15 genes were co‐upregulated in OPN highly expression CRC and a list of candidate drugs that may have potential to reverse the secreted phosphoprotein 1 ( SPP1 ) gene signature by connectivity mapping. In summary, OPN is a potential prognostic indicator and therapeutic target for colon cancer.