Integration of organic electrochemical transistors and immuno-affinity membranes for label-free detection of interleukin-6 in the physiological concentration range through antibody–antigen recognition. Issue 33 (13th August 2018)

• Record Type: Journal Article
• Authors: Gentili, Denis; D'Angelo, Pasquale; Militano, Francesca; Mazzei, Rosalinda; Poerio, Teresa; Brucale, Marco; Tarabella, Giuseppe; Bonetti, Simone; Marasso, Simone L.; Cocuzza, Matteo; Giorno, Lidietta; Iannotta, Salvatore; Cavallini, Massimiliano
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 33(2018)
• Page Start: 5400
• Page End: 5406
• Publication Date: 2018-08-13
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb01697f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A label-free immunosensor based on an organic electrochemical transistor integrated with an immuno-affinity membrane for cytokine detection at physiologically relevant concentrations is reported. Abstract : We demonstrate the label-free and selective detection of interleukin-6 (IL-6), a key cell-signaling molecule in biology and medicine, by integrating an OECT with an immuno-affinity regenerated cellulose membrane. The objective of the membrane is to increase the local concentration of IL-6 at the sensing electrode and, thereby, enhance the device response for concentrations falling within the physiological concentration range of cytokines. The OECT gate electrode is functionalized with an oligo(ethylene glycol)-terminated self-assembled alkanethiolate monolayer (SAM) for both the immobilization of anti IL-6 antibodies and the inhibition of non-specific biomolecule binding. The OECT gate/electrolyte interface is exploited for the selective detection of IL-6 through the monitoring of antigen–antibody binding events occurring at the gate electrode.