In vitro mimicking the morphology of hepatic lobule tissue based on Ca-alginate cell sheets. (21st February 2018)

• Record Type: Journal Article
• Authors: Liu, Zeyang; Lu, Minmin; Takeuchi, Masaru; Yue, Tao; Hasegawa, Yasuhisa; Huang, Qiang; Fukuda, Toshio
• Is Part Of: Biomedical materials. Volume 13:Number 3(2018:Jun.)
• Publication Date: 2018-02-21
• Subjects: Ca-alginate hydrogel -- electrodeposition -- mimic of liver tissue -- cell sheets; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/aaa4c4
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: Artificial cell sheets are commonly utilized as buiding blocks for tissue engineering. We propose a novel approach in the fabrication of Ca-alginate gel sheets, embedded with liver cells (RLC-18) in order to mimic liver lobule tissue. Ca-alginate sheets with hepatic lobule-shaped patterns were deposited onto a micro-electrode device using electrodeposition. Viability of embedded cells was ensured to exceed 80%. Cell morphology and biofunctionality were monitored during the one-week culture period and results compared with those of traditional 2D culture. In addition, we detached cell sheets from the electrode substrate and stacked them into a 3D multi-layered structure to mimic the morphology of liver lobule tissue.