Silk fibroin/amniotic membrane 3D bi-layered artificial skin. (20th February 2018)

• Record Type: Journal Article
• Authors: Gholipourmalekabadi, Mazaher; Samadikuchaksaraei, Ali; Seifalian, Alexander M; Urbanska, Aleksandra M; Ghanbarian, Hossein; Hardy, John G; Omrani, Mir Davood; Mozafari, Masoud; Reis, Rui L; Kundu, Subhas C
• Is Part Of: Biomedical materials. Volume 13:Number 3(2018:Jun.)
• Publication Date: 2018-02-20
• Subjects: scaffold -- skin -- regenerative medicine -- human amniotic membrane -- silk fibroin; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/aa999b
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce β -sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for skin regeneration.