Characterization of gelatin and chitosan scaffolds cross-linked by addition of dialdehyde starch. (15th December 2017)

• Record Type: Journal Article
• Authors: Kaczmarek, B; Sionkowska, A; Monteiro, F J; Carvalho, A; Łukowicz, K; Osyczka, A M
• Is Part Of: Biomedical materials. Volume 13:Number 1(2018:Feb.)
• Publication Date: 2017-12-15
• Subjects: chitosan -- gelatin -- dialdehyde starch -- scaffolds -- SaOS-2 cells -- attachment -- proliferation; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1088/1748-605X/aa8910
• Languages: English
• ISSNs: 1748-6041

Abstract

Abstract: In this study the influence of the addition of dialdehyde starch on the properties of scaffolds based on gelatin and chitosan obtained by the freeze-drying method was investigated. In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells on the obtained scaffolds was examined. Chitosan and gelatin were mixed in different weight ratios (75/25, 50/50, 25/75) with 1, 2 and 5 wt% addition of dialdehyde starch. The obtained scaffolds were subjected to mechanical testing, infrared spectroscopy, swelling measurements, low-pressure porosimetry and zeta potential measurement. Internal material structures were observed by scanning electron microscopy. The results showed that the cross-linking process occurred after the addition of dialdehyde starch and resulted in increased mechanical strength, swelling properties, zeta potential and porosity of studied materials. The attachment of SaOS-2 cells to all modified materials was better compared to an unmodified control and the proliferation of these cells was markedly increased on modified scaffolds.