Mitochondrial dynamics and mitophagy are necessary for proper invasive growth in rice blast. (20th June 2019)

• Record Type: Journal Article
• Authors: Kou, Yanjun; He, Yunlong; Qiu, Jiehua; Shu, Yazhou; Yang, Fan; Deng, YiZhen; Naqvi, Naweed I.
• Is Part Of: Molecular plant pathology. Volume 20:Number 8(2019)
• Page Start: 1147
• Page End: 1162
• Publication Date: 2019-06-20
• Subjects: Atg24 -- Dnm1 -- Fzo1 -- Magnaporthe oryzae–rice interaction -- mitochondrial fusion and fission -- mitophagy -- rice blast; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12822
• Languages: English
• ISSNs: 1464-6722

Abstract

Summary: Magnaporthe oryzae causes blast disease, which is one of the most devastating infections in rice and several important cereal crops. Magnaporthe oryzae needs to coordinate gene regulation, morphological changes, nutrient acquisition and host evasion in order to invade and proliferate within the plant tissues. Thus far, the molecular mechanisms underlying the regulation of invasive growth in planta have remained largely unknown. We identified a precise filamentous‐punctate‐filamentous cycle in mitochondrial morphology during Magnaporthe –rice interaction. Interestingly, disruption of such mitochondrial dynamics by deletion of genes regulating either the mitochondrial fusion (MoFzo1) or fission (MoDnm1) machinery, or inhibition of mitochondrial fission using Mdivi‐1 caused significant reduction in M. oryzae pathogenicity. Furthermore, exogenous carbon source(s) but not antioxidant treatment delayed such mitochondrial dynamics/transition during invasive growth. In contrast, carbon starvation induced the breakdown of the mitochondrial network and led to more punctate mitochondria in vitro . Such nutrient‐based regulation of organellar dynamics preceded MoAtg24‐mediated mitophagy, which was found to be essential for proper biotrophic development and invasive growth in planta . We propose that precise mitochondrial dynamics and mitophagy occur during the transition from biotrophy to necrotrophy and are required for proper induction and establishment of the blast disease in rice.