Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation. (18th May 2019)

• Record Type: Journal Article
• Authors: Li, Yan; Héloir, Marie‐Claire; Zhang, Xun; Geissler, Mareen; Trouvelot, Sophie; Jacquens, Lucile; Henkel, Marius; Su, Xin; Fang, Xuewen; Wang, Qi; Adrian, Marielle
• Is Part Of: Molecular plant pathology. Volume 20:Number 8(2019)
• Page Start: 1037
• Page End: 1050
• Publication Date: 2019-05-18
• Subjects: Bacillus subtilis -- defence -- downy mildew -- fengycin -- lipopeptide -- Plasmopara viticola -- surfactin; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12809
• Languages: English
• ISSNs: 1464-6722

Abstract

Summary: Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola, the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High‐performance thin‐layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew.