Silver nanoparticle-mediated cellular responses in isolated primary Sertoli cells in vitro. (June 2018)

• Record Type: Journal Article
• Authors: Habas, Khaled; Brinkworth, Martin H.; Anderson, Diana
• Is Part Of: Food and chemical toxicology. Volume 116:Part B(2018)
• Page Start: 182
• Page End: 188
• Publication Date: 2018-06
• Subjects: Silver nanoparticles -- Sertoli cell -- DNA damage -- Oxidative stress -- Gene expression -- Endogenous antioxidant enzymes; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.04.030
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The present study explored the mechanism of cytotoxic and genotoxic effects of AgNPs on a primary culture of mouse Sertoli cells in vitro . To understand the possible molecular mechanisms of testicular lesions following exposure to AgNPs, isolated Sertoli cells were exposed to 5, 10, or 15 μg/ml. DNA damage in the Comet assay and apoptosis in the TUNEL assay were evaluated. The mRNA expression of p53 and bcl-2 genes and their proteins involved in apoptosis was also investigated. The antioxidant status of treated Sertoli cells was determined by measuring superoxide dismutase (SOD-1), catalase (CAT) and glutathione peroxidase (GPX-1) using quantitative polymerase chain reaction (qPCR). The superoxide anions were detected using the nitroblue tetrazolium (NBT) reduction assay. Results indicated that AgNP exposure causes increased oxidative stress levels. The activation of p53, repression of bcl-2 and reduction of endogenous antioxidant enzymes were also involved in these mechanistic pathways, leading to reduced cell numbers and cell detachment. Graphical abstract: Highlights: AgNPs entered Sertoli cells and caused severe testicular oxidative damage and/or apoptosis. Exposure of the Sertoli cells to AgNPs produced significant increases in superoxide anions. Exposure to AgNPs activated p53, repressed bcl-2 and reduced endogenous antioxidant enzymes. The male reproductive system can be disrupted by exposure of somatic cells to AgNPs.