Fluorogenic substrates for the detection of saliva. (December 2017)

• Record Type: Journal Article
• Authors: Gooch, James; Chua, Chang Rong; Abbate, Vincenzo; Frascione, Nunzianda
• Is Part Of: Forensic science international. Volume 6(2017)
• Page Start: e565
• Page End: e567
• Publication Date: 2017-12
• Subjects: Fluorogenic substrates -- Saliva detection -- Body fluids; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2017.09.218
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: The potential of fluorogenic substrates to detect and identify human saliva in situ was first explored using commercially available substrate Boc-VPR-AMC. The substrate was applied to a range of saliva dilutions deposited on glass microscope slides. A positive fluorescence response was observed immediately after application against each deposit up to a 1:8 dilution. In an attempt to improve assay sensitivity, a novel substrate Ac-VPR-Rho110-Ac, utilising a Rhodamine-110 fluorophore, was prepared using a solid-phase peptide synthesis protocol previously reported by our research group. Application of Ac-VPR-Rho110-Ac to saliva deposits demonstrated a successful increase in assay sensitivity limits, allowing stains of up to 1 in 128 dilution to be detected.