Automation of library preparation using Illumina ForenSeq kit for routine sequencing of casework samples. (December 2017)

• Record Type: Journal Article
• Authors: Laurent, F.X.; Ausset, L.; Clot, M.; Jullien, S.; Chantrel, Y.; Hollard, C.; Pene, L.
• Is Part Of: Forensic science international. Volume 6(2017)
• Page Start: e415
• Page End: e417
• Publication Date: 2017-12
• Subjects: Multiple parallel sequencing -- ForenSeq -- Illumina -- Automation -- Hamilton -- Forensic genomics; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2017.09.156
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: Massively Parallel Sequencing (MPS) technologies are called upon to play a major role in forensic genomics in the next few years. However, library preparation protocols contain numerous steps which can complexify casework analysis in routine. Implementation of a MPS analytical workflow, in a casework laboratory, requires automation to guarantee a full sample tracking and to minimize contamination risks. In this study, we present the development and validation of a fully automated workflow using Illumina ForenSeq DNA signature Prep Kit on a NGS STARlet HID Illumina platform. Automation greatly helps with library preparation reproducibility and significantly reduces hands-on time. We are confident that automation of MPS technologies will enhance forensic laboratory to adopt these powerful analytical tools determinant for the future of the forensic genomics field.