Analyzing an "Off-Ladder" allele at DXS10135 from the AGCU X19 STR kit. (December 2017)

• Record Type: Journal Article
• Authors: Li, Jienan; Deng, Chuncao; Luo, Haibo; Song, Feng; Hou, Yiping
• Is Part Of: Forensic science international. Volume 6(2017)
• Page Start: e92
• Page End: e93
• Publication Date: 2017-12
• Subjects: X-STRs -- Off-ladder -- AGCU X19 STR Kit; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2017.09.045
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: X chromosome STRs (X-STRs) have been proven to be an important tool for some complex kinship analyses in recent years. A novel 19 X-STRs multiplex system named as AGCU X19 STR Kit was developed and widely used for population genetics as well as forensic case works. In this study an "Off-ladder" allele between allele 21 and allele 21.1 at DXS10135 has been observed in AGCU X19 STR Kit. The "Off-ladder" allele was about 0.4 bases longer than the allele 21 of allelic ladder of DXS10135 in AGCU X19 STR Kit. To avoid DNA profile misunderstanding or mislabeling, "Off-ladder" allele at DXS10135 was analyzed by sequencing and detected with Investigator Argus X-12 Kit, respectively. Our results showed this "Off-ladder" allele was allele 21 at DXS10135. It was caused by inaccurate allele 21 of allelic ladder of DXS10135 in AGCU X19 STR Kit.