Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator. (10th April 2018)

• Record Type: Journal Article
• Authors: Swarbrick, Samantha; Efimov, Igor; Ostanin, Victor P.; Klenerman, David; Stolzing, Alexandra; Ghosh, Sourav K.
• Is Part Of: Electrochimica acta. Volume 269(2018)
• Page Start: 526
• Page End: 533
• Publication Date: 2018-04-10
• Subjects: Electrochemical quartz crystal microbalance -- Acoustic detection of biomarkers -- Anharmonic oscillations -- Single-stranded DNA hybridization -- Electrochemical impedance; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2018.03.014
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: The paper describes a sensor for single-stranded DNA (ssDNA) biomarker based on anharmonic acoustic signals arising during hybridization with complementary thiolated ssDNA functionalised on the gold electrode of an electrochemical quartz crystal resonator. The steps of sensor preparation and hybridization are carried out in an electrochemical microfluidic flowcell. While the electrochemical impedance spectroscopy does not allow a definitive interpretation, the changes in resonance frequency and third Fourier harmonic current of the resonator on actuation at the fundamental mode indicate formation of a flexibly bound layer. The functionalization and hybridization steps monitored by the anharmonic detection technique (ADT) are described with a simple model based on Duffing nonlinear equation.