Validation of ABI 3500xL Genetic Analyzer after decommissioned and recommissioned at new premises. (December 2017)

• Record Type: Journal Article
• Authors: Iyavoo, S.; Draus-Barini, J.; Haizel, T.
• Is Part Of: Forensic science international. Volume 6(2017)
• Page Start: e542
• Page End: e543
• Publication Date: 2017-12
• Subjects: DNA -- ABI 3500xL Genetic Analyzer -- Capillary electrophoresis -- Validation -- Accreditation -- Concordance -- Sensitivity -- Repeatability; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2017.09.197
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: This validation was aimed to demonstrate that there is no effect on the Applied Biosystems ® 3500xL Genetic Analyzer (Thermo Fisher Scientific ®, UK) after decommissioned and recommissioned at new premises. Same sample sets were tested on the ABI 3500xL Genetic Analyzer at both old and new premises using current accredited procedures. The results showed genotyping concordance which means using the ABI 3500xL Genetic Analyzer at the new premises provides no risk regarding the accuracy, precision and reproducibility of the results and also showed a very good performance on sensitivity, specificity, repeatability and good profile quality based on the assessment of locus peak balance and peak height.