Microsatellite markers for Corybas (Orchidaceae) species in New Zealand. (9th November 2018)

• Record Type: Journal Article
• Authors: Van Etten, Megan L.; Lehnebach, Carlos A.; Pearson, Sofie M.; Robertson, Alastair W.; Tate, Jennifer A.
• Is Part Of: Applications in plant sciences. Volume 6:Number 11(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-11-09
• Subjects: Corybas -- Corybas obscurus -- microsatellite -- New Zealand -- Orchidaceae -- spider orchid; Plants -- Periodicals; Plant physiology -- Periodicals; Plant Physiological Phenomena; Plant physiology; Plants; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 580
• DOI: 10.1002/aps3.1192
• Languages: English
• ISSNs: 2168-0450

Abstract

Abstract : Premise of the Study: Microsatellite markers were developed for New Zealand species of Corybas (Orchidaceae) to investigate population genetics and species delimitation. Methods and Results: From sequencing a total genomic DNA library (using Illumina MiSeq), we developed 22 microsatellite markers for C. obscurus . The di‐ and trinucleotide repeat loci were initially trialed on individuals representing seven Corybas taxa ( C . "rimutaka, " C. confusus, C. hypogaeus, C. macranthus, C. obscurus, C. trilobus, and C. walliae ) and had one to eight alleles per locus. Twelve polymorphic markers were further tested on six Corybas populations from three of the seven taxa ( C. obscurus, C . "rimutaka, " and C. trilobus ). Observed and expected heterozygosities ranged from 0–1 and 0–0.859, respectively. The utility of these 12 loci was further validated in five related Corybas species ( C. hypogaeus, C. obscurus, C. vitreus, C. walliae, and C . "rimutaka"; 38 individuals) representing populations from across the North and South Islands. The average value for genetic diversity among populations ( F ST ) of 0.439 shows differentiation among species. Conclusions: These markers will be useful for future studies aimed at delimiting species boundaries and examining the genetic diversity of the New Zealand Corybas species.