Postembryonic Fish Brain Proliferation Zones Exhibit Neuroepithelial‐Type Gene Expression Profile. (14th March 2017)

• Record Type: Journal Article
• Authors: Dambroise, Emilie; Simion, Matthieu; Bourquard, Thomas; Bouffard, Stéphanie; Rizzi, Barbara; Jaszczyszyn, Yan; Bourge, Mickaël; Affaticati, Pierre; Heuzé, Aurélie; Jouralet, Julia; Edouard, Joanne; Brown, Spencer; Thermes, Claude; Poupon, Anne; Reiter, Eric; Sohm, Frédéric; Bourrat, Franck; Joly, Jean‐Stéphane
• Is Part Of: Stem cells. Volume 35:Number 6(2017:Jun.)
• Page Start: 1505
• Page End: 1518
• Publication Date: 2017-03-14
• Subjects: Neuroepithelial cell -- DNA repair -- Cell cycle -- RNA seq -- Optic tectum -- Microcephaly; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2588
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: In mammals, neuroepithelial cells play an essential role in embryonic neurogenesis, whereas glial stem cells are the principal source of neurons at postembryonic stages. By contrast, neuroepithelial‐like stem/progenitor (NE) cells have been shown to be present throughout life in teleosts. We used three‐dimensional (3D) reconstructions of cleared transgenic wdr12 :GFP medaka brains to demonstrate that this cell type is widespread in juvenile and to identify new regions containing NE cells. We established the gene expression profile of optic tectum (OT) NE cells by cell sorting followed by RNA‐seq. Our results demonstrate that most OT NE cells are indeed active stem cells and that some of them exhibit long G2 phases. We identified several novel pathways (e.g., DNA repair pathways) potentially involved in NE cell homeostasis. In situ hybridization studies showed that all NE populations in the postembryonic medaka brain have a similar molecular signature. Our findings highlight the importance of NE progenitors in medaka and improve our understanding of NE‐cell biology. These cells are potentially useful not only for neural stem cell studies but also for improving the characterization of neurodevelopmental diseases, such as microcephaly. Stem Cells 2017;35:1505–1518 Abstract : We performed an enhancer screen in a locus composed of two medaka genes in tandem having a preferential expression in neuroepithelium. Thus, we isolated a driver for cells that we demonstrated to have cytological features of neuroepithelial cells. (A) Analysis of histological sections and 3D images from clarified brains of transgenic fish indicated that neuroepithelial‐based neurogenesis may be present in almost all the proliferation zones of the fish brain. Our study almost also points to the intriguing possibility that neuroepithelial cells exhibit long G2 phases. (B) RNA seq from optic tectum GFP+ sorted cells revealed neuroepithelium marker genes, some of which are orthologous to mammalian neuroepithelium markers. Many genes are associated to ribosome biogenesis, proliferation or DNA repair.