Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples. Issue 6 (2nd November 2017)

• Record Type: Journal Article
• Authors: Liu, Liqiang; Chen, Yanni; Song, Shanshan; Zheng, Qiankun; Wu, Xiaoling; Kuang, Hua
• Is Part Of: Food and agricultural immunology. Volume 28:Issue 6(2017)
• Page Start: 1283
• Page End: 1292
• Publication Date: 2017-11-02
• Subjects: Immunochromatographic lateral flow strip test -- natamycin -- milk samples -- yoghurt samples; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2017.1337084
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: An immunochromatographic lateral flow strip test was developed for the detection of natamycin (Nata) residues in milk and cheese samples. Monoclonal antibody (mAb) against Nata was produced with a half maximal inhibitory concentration of 1.85 μg L −1 . MAb conjugated with gold nanoparticles was the detection reagent. Nata conjugated with ovalbumin via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/ N -hydroxysuccinimide method immobilized on a nitrocellulose membrane was the capture reagent. This semi-quantitative method required only 15 min to complete. The optimum concentrations of coating antigen and mAb were 0.15 µg mL −1 and 0.2 µg mL −1, respectively. Based on an optical density scanner, the visual limit of detection of Nata was 5 μg L −1 and 10 μg kg −1 in milk and yoghurt samples, respectively.