Development and characterization of 10 novel polymorphic microsatellite loci in Homatula potanini (Günther, 1896). (6th June 2019)

• Record Type: Journal Article
• Authors: Li, Weitao; Liao, Xiaolin; Xu, Nian; Que, Yanfu; Xiong, Meihua; Zhu, Bin; Shao, Ke
• Is Part Of: Journal of applied ichthyology. Volume 35:Number 4(2019:Aug.)
• Page Start: 1025
• Page End: 1028
• Publication Date: 2019-06-06
• Subjects: Fishes -- Periodicals; Fish culture -- Periodicals; Fisheries -- Periodicals; Ichthyology -- Periodicals; 597
• DOI: 10.1111/jai.13926
• Languages: English
• ISSNs: 0175-8659

Abstract

Abstract: Homatula potanini is a genus Paracobitis which belongs to the family Cobitidae, subfamily Nemacheilinae. We first isolated ten novel microsatellite DNA loci using the FIASCO protocol. The number of observed alleles per locus ranged from 5 to 14. The observed heterozygosity and expected heterozygosity ranged from 0.3750 to 0.9375 and 0.4028 to 0.8819 respectively. The polymorphic information content ranged from 0.3515 to 0.8556 ( M = 0.6170). No significant linkage disequilibrium was detected among the loci. In the present study, we have identified 10 new markers for H. potanini . These loci should provide sufficient levels in the evaluation of genetic diversity and designing effective conservation programs of H. potanini .