Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Shim, Man; Yoon, Hong; Lee, Sangmin; Jo, Mun; Park, Jooho; Kim, Jong-Ho; Jeong, Seo; Kwon, Ick; Kim, Kwangmeyung
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 15
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-16653-2
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract Apoptosis is one of the most important intracellular events in living cell, which is a programmed cell death interrelated with caspase enzyme activity for maintaining homeostasis in multicellular organisms. Therefore, direct apoptosis imaging of living cells can provide enormous advantages for diagnosis, drug discovery, and therapeutic monitoring in various diseases. However, a method of direct apoptosis imaging has not been fully validated, especially for live cells inin vitro andin vivo . Herein, we developed a new apoptosis imaging technology via a direct visualization of active caspase-3/-7 activity in living cells. For this, we synthesized a caspase-3/-7-specific cleavable peptide (KGDEVD) conjugated triacetylated N-azidoacetyl-D-mannosamine (Apo-S-Ac3 ManNAz), wherein the Apo-S-Ac3 ManNAz can be cleaved by the active caspase-3/-7 in live apoptotic cells and the cleaved Ac3 ManNAz molecules can further generate targetable azido groups (N3 ) on the living cell surface. Importantly, the azido groups on the apoptotic tumor cells could be visualized with Cy5.5-conjugated dibenzylcyclooctyne (DBCO-Cy5.5) via bioorthogonal click chemistryin vitro cell culture condition andin vivo tumor-bearing mice. Therefore, our Apo-S-Ac3 ManNAz can be utilized for the further applications in tumor therapy as a monitoring tool for anticancer efficacy and optimization of anticancer new drugs in cell culture system and in tumor-bearing mice.