Ziziphus spina-christi fruit extract suppresses oxidative stress and p38 MAPK expression in ulcerative colitis in rats via induction of Nrf2 and HO-1 expression. (May 2018)

• Record Type: Journal Article
• Authors: Almeer, Rafa S.; Mahmoud, Sahar M.; Amin, Hatem K.; Abdel Moneim, Ahmed E.
• Is Part Of: Food and chemical toxicology. Volume 115(2018)
• Page Start: 49
• Page End: 62
• Publication Date: 2018-05
• Subjects: AcOH acetic acid -- ARE antioxidant responsive element -- Cas-3 caspase-3 -- CAT catalase -- CMIS colonic mucosal injury score -- COX cyclooxygenase -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- GRd glutathione reductase -- GPx glutathione peroxidase -- H&E hematoxylin and eosin -- HDI histopathological damage index -- HO-1 heme oxygenase-1 -- IBD inflammatory bowel disease -- IL-1β interleukin-1β -- iNOS inducible nitric oxide synthase -- LPO lipid peroxidation -- MAPK mitogen-activated protein kinase -- MDA malondialdehyde -- MLZ mesalazine -- MPO myeloperoxidase -- NADH reduced nicotinamide adenine dinucleotide -- NBT nitroblue tetrazolium -- NF-кB nuclear factor kappa-B -- Nrf2 nuclear factor (erythroid-derived 2)-like 2 -- PAS/AB periodic acid-Schiff/Alcian blue -- PMS phenazine methosulfate -- SOD superoxide dismutase -- TBA thiobarbituric acid -- TNF-α tumor necrosis factor-α -- UC ulcerative colitis -- VEGF vascular endothelial growth factor -- ZFE Ziziphus spina-christi fruit extract; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.03.002
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: In this study, we aimed to evaluate the anti-inflammatory and protective effects of Ziziphus spina-christi fruit extract (ZFE) against acetic acid (AcOH)-induced colitis in rats. Before a single AcOH instillation, ZFE (100, 200, and 400 mg/kg/day) was administered for 5 days by oral gavage. Pretreatment with ZFE at different doses suppressed the spread of inflammation and inhibited mucosal damage; in addition, it reduced ulcer size and mitigated colitis markers. Administration of ZFE (400 mg/kg) resulted in a greater reduction of inflammatory colonic injury than that after reference drug, mesalazine (MLZ), administration. In addition, ZFE not only histopathologically ameliorated AcOH-induced colitis but also restored the balance between the oxidants and antioxidants. Furthermore, ZFE effectively modulated the mRNA expression of redox-sensitive transcription factors, such as nuclear factor (erythroid-derived 2)-like 2 and heme oxygenase-1, downregulated the expression of p38 mitogen-activated protein kinase, and upregulated that of vascular endothelial growth factor A and interleukin-1β in AcOH-induced colitis in rats. In conclusion, our results suggested that ZFE could prevent the development of chronic experimental colitis in rats; therefore, it could be considered as an alternative and/or additive therapeutic approach for the management of inflammatory bowel disease. Graphical abstract: Highlights: Ulcerative colitis (UC) leads to colonic inflammation and oxidative stress. Zizyphus spina - christi inhibits UC in rats. Zizyphus spina-christi suppresses MAPKs and NF-κB signaling pathways. ZSCLE has antiinflammatory and antioxidant properties.