Illumina-MiSeq analysis of fungi in acid lime roots reveals dominance of Fusarium and variation in fungal taxa. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Al-Sadi, Abdullah; Kazerooni, Elham
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 7
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-35404-5
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract A study was conducted to analyze fungal diversity in the roots of acid lime (Citrus aurantifolia ) collected from Oman, a semi-arid country located in the South Eastern part of the Arabian Peninsula. MiSeq analysis showed theAscomycota andSordariomycetes were the most abundant phylum and class in acid lime roots, respectively.Glomeromycota, Basidiomycota andMicrosporidia were the other fungal phyla, whileGlomeromycetes and some other classes belonging toAscomycota andBasidiomycota were detected at lower frequencies. The genusFusarium was the most abundant in all samples, making up 46 to 95% of the total reads. Some fungal genera of Arbuscular mycorrhizae and nematophagous fungi were detected in some of the acid lime roots. Analysis of the level of fungal diversity showed that no significant differences exist among groups of root samples (from different locations) in their Chao richness and Shannon diversity levels (P < 0.05). Principle component analysis of fungal communities significantly separated samples according to their locations. This is the first study to evaluate fungal diversity in acid lime roots using high throughput sequencing analysis. The study reveals the presence of various fungal taxa in the roots, dominated byFusarium species and including some mycorrhizae and nematophagous fungi.