Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation. Issue 1 (December 2017)

• Record Type: Journal Article
• Authors: Valdez, Chris; Barnes, Ronald; Roth, Caleb; Moen, Erick; Throckmorton, Graham; Ibey, Bennett
• Is Part Of: Scientific reports. Volume 7:Issue 1(2017)
• Page Start: 1
• Page End: 12
• Publication Date: 2017-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-017-16142-6
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed "bipolar cancellation " (BPC ). The predominant BP nsEP parameters that induceBPC consist of a positive polarity (↑) front pulse followed by the delivery of a negative polarity (↓) back pulse of equal voltage and width; thereby the duration is twice a UP nsEP exposure. We tested theseBPC parameters, and discovered that a BP nsEP with symmetrical pulse widths is not required to generateBPC . For example, our data revealed the physiological response initiated by a ↑900 nsEP exposure can becancelled by a second pulse that is a third of its duration. However, we observed a complete loss ofBPC from a ↑300 nsEP followed by a ↓900 nsEP exposure. Spatiotemporal analysis revealed these asymmetrical BP nsEP exposures generate distinct local YO-PRO®-1 uptake patterns across the plasma membrane. From these findings, we generated a conceptual model that suggestsBPC is a phenomenon balanced by localized charging and discharging events across the membrane.