Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase. Issue 2 (2001)

• Record Type: Journal Article
• Authors: Svinarich, David M.; Zaher, Fadi M.; Holmdahl, Lena; Chegini, Nasser; Gonik, Bernard; Diamond, Michael P.
• Is Part Of: Infectious diseases in obstetrics and gynecology. Volume 9:Issue 2(2001)
• Page Start: 113
• Page End: 116
• Publication Date: 2001
• Subjects: Generative organs, Female -- Infections -- Periodicals; Communicable diseases in pregnancy -- Periodicals; 618.142005
• DOI: 10.1155/S1064744901000199
• Languages: English
• ISSNs: 1064-7449

Abstract

Abstract : Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 ×10 -4, ≤ 3.12 ×10 -4, 6.24 ×10 -4 and 2.5 ×10 -3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 ×10 -1 and 6.25 ×10 -2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.