Development of an image biosensor based on an optogenetically engineered cell for visual prostheses. Issue 28 (11th June 2019)

• Record Type: Journal Article
• Authors: Li, Gongxin; Wang, Feifei; Yang, Wenguang; Yang, Jia; Wang, Yuechao; Wang, Wenxue; Liu, Lianqing
• Is Part Of: Nanoscale. Volume 11:Issue 28(2019)
• Page Start: 13213
• Page End: 13218
• Publication Date: 2019-06-11
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c9nr01688k
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : An image biosensor is built by an optogenetically engineered cell and used to acquire high-resolution images for the development of the new generation of visual prostheses. Abstract : Visual prostheses provide blind patients with artificial vision via electrical stimulation of surviving visual cells resulting in partial restoration of vision in many patients. However, high-resolution visual perception, long-term biocompatibility and safety remain the significant challenges of existing visual prostheses. Here, we present a novel method to develop a new visual prosthesis using living cells as integrated electronics and implantable microelectrodes. The living cells modified with channelrhodopsin-2 showed excellent light-sensitive properties and encoded image information with cellular deformations triggered by light stimulation. The photoresponsive properties of the cells were determined using a single pixel imaging system, which indicated that the cells can act as a good light-sensitive biosensor. Additionally, the imaging feasibility of the cells was further validated through successful and clear imaging of several object scenes using the same system. This work represents a step toward the design and use of living cells as an image biosensor for the development of a new generation of high-resolution visual prostheses.