A biomimetic tarso-conjunctival biphasic scaffold for eyelid reconstruction in vivo. (24th June 2019)

• Record Type: Journal Article
• Authors: Xu, Peifang; Gao, Qi; Feng, Xue; Lou, Lixia; Zhu, Tiepei; Gao, Changyou; Ye, Juan
• Is Part Of: Biomaterials science. Volume 7:Number 8(2019)
• Page Start: 3373
• Page End: 3385
• Publication Date: 2019-06-24
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c9bm00431a
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : A biphasic scaffold with bionic structure mimicking tarsus and conjunctiva was manufactured and evaluated in vitro and in vivo . Abstract : Conventional 3D porous scaffolds used as tarsal plate substitute may cause corneal irritation and conjunctival mucoid discharge, and even lead to blindness and cicatricial blepharon deformities. In this study, collagen/chitosan (Col/CS) sponges with thickness of 240 μm, 466 μm, and 724 μm were composited onto poly(propylene fumarate)- co -2-hydroxyethyl methacrylate (PPF–HEMA) polymer networks to obtain the corresponding biphasic scaffolds, which simulate the natural anatomy of posterior lamella of eyelid. These three scaffolds exhibited a porous structure with porosity of ∼90%, simulated elastic modulus, appropriate degradation rate and good biocompatibility. Composited with Col/CS sponge of difference thickness, the scaffolds induced different cellular behaviors such as proliferation, distribution and stratification, by regulating the mechanical properties cells sensed as effective modulus. In a rabbit tarso-conjunctival defect model, the grafted biphasic scaffolds promoted re-epithelization with functional regenerated conjunctiva. Hence, the biphasic composite scaffolds may be a promising substitute for tarso-conjunctival repair.