Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia. (October 2019)

• Record Type: Journal Article
• Authors: Ekawasti, Fitrine; Nurcahyo, Wisnu; Wardhana, April Hari; Shibahara, Tomoyuki; Tokoro, Masaharu; Sasai, Kazumi; Matsubayashi, Makoto
• Is Part Of: Parasitology international. Volume 72(2019)
• Publication Date: 2019-10
• Subjects: Cattle -- Eimeria bovis -- Eimeria zuernii -- Indonesia -- Java Island; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2019.101927
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: Gastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of Indonesia, fecal examinations by the Whitlock and sugar flotation methods and molecular surveys were conducted to reveal the prevalence of 6 Eimeria spp. As a result of morphological screening using Whitlock methods and sugar flotation, Eimeria spp. prevalences of 9.4% and 52.3% were confirmed, respectively. The prevalence was higher in younger cattle [under 1 year (63.9%), 1–2 years (75.0%) and more than in 2 year old cattle (42.3%)]. The prevalences of identified species were as follows: 10.4% for E. bovis, 2.8% for E. ellipsoidalis, 2.1% for E. alabamensis, 1.4% for E. zuernii, 1.1% for E. auburnensis, and 0.4% for E. cylindrica . Moreover, prevalences of 12.8% for Strongyloides papillosus, 7.3% for Trichuris globulosa, and 0.3% for Capillaria bovis were detected. Although the average number of oocysts per gram of feces was <100 among the positive samples, and cases of heavy infection were limited, the endemicity of these pathogenic Eimeria species among farms in Indonesia should be noted. Graphical abstract: Unlabelled Image Highlights: In our fecal examinations of Eimeria spp., all of examined 14 districts in Java Island were positive for Eimeria spp. The prevalence was higher in young cattle under 2 years than those of over 2 years. By PCR analyses, E. bovis was found to be predominant among the detected species. The endemicity of the pathogenic Eimeria species among farms in Indonesia should be noted.