A two-stage model with nitrogen and silicon limitation enhances lipid productivity and biodiesel features of the marine bloom-forming diatom Skeletonema costatum. (October 2019)

• Record Type: Journal Article
• Authors: Gao, Guang; Wu, Min; Fu, Qianqian; Li, Xinshu; Xu, Juntian
• Is Part Of: Bioresource technology. Volume 289(2019)
• Publication Date: 2019-10
• Subjects: Biochemical -- Biodiesel -- Diatom -- Lipid -- Fatty acid -- Photosynthesis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121717
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: N-Si-limitation increased lipid productivity by 76.7% for 6 h induction. N-Si-limitation increased lipid productivity by 113.7% for 12 h induction. N-Si-limitation enhanced the proportions of SFA and MUFA but reduced PUFA. N-Si-limitation reduced iodine value by 33.7% for 6 h and 45.6% for 24 h induction. N-Si-limitation increased cetane number by 6.4% for 6 h and 21.6% for 24 h induction. Abstract: To enhance biodiesel production and quality from a bloom-forming diatom Skeletonema costatum, a two-stage model, in which cells were cultured in nutrient replete conditions first and then transferred to nutrient limitation conditions, was explored. Compared to one-stage model, nutrient limitation in the second stage significantly increased lipid content in spite of decreasing growth; consequently, Si-limitation and N-Si-limitation respectively increased lipid productivity by 37.6% and 76.7% for 6 h induction, and 42.8% and 113.7% for 12 induction. Nutrient limitation enhanced the proportions of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) but reduced polyunsaturated fatty acid (PUFA). Therefore, N-Si-limitation reduced iodine value by 33.7% and 45.6% but increased cetane number by 6.4% and 21.6% for 6 and 24 h induction, respectively. These findings indicate that the two-stage model with N-Si-limitation can enhance lipid productivity as well as biodiesel quality from diatoms.