Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii С-134. (15th July 2019)

• Record Type: Journal Article
• Authors: Sizova, Olga V.; Shashkov, Alexander S.; Toukach, Philip V.; Knirel, Yuriy A.; Shaikhutdinova, Rima Z.; Ivanov, Sergei A.; Kislichkina, Angelina A.; Dentovskaya, Svetlana V.
• Is Part Of: Carbohydrate research. Volume 481(2019)
• Page Start: 9
• Page End: 15
• Publication Date: 2019-07-15
• Subjects: Yersinia kristensenii -- O-specific polysaccharide -- O-antigen -- Lipopolysaccharide -- Glycerol 1-phosphate -- Bacterial polysaccharide structure; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2019.06.001
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: This structure is related to those of other Y. kristensenii O-polysaccharides studied earlier. The O-antigen gene cluster of Y. kristensenii С-134 was analyzed and found to be consistent with the O-polysaccharide structure established. Graphical abstract: Image 1 Highlights: Structure of the O-polysaccharide of Yersinia kristensenii C-134 was established. The O-polysaccharide has a glycerol teichoic acid-like structure. Dephosphorylation of the O-polysaccharide afforded a glycerol-linked hexasaccharide. The O-antigen gene cluster was found to be consistent with the O-polysaccharide structure.