Determination of trichlorfon residues in vegetables using a quantum dot-labeled biomimetic immunoassay method followed by capillary electrophoresis. Issue 6 (2nd November 2017)

• Record Type: Journal Article
• Authors: Jiang, Mingdi; Wu, Shang; Xu, Longhua; Qiao, Xuguang; Xu, Zhixiang
• Is Part Of: Food and agricultural immunology. Volume 28:Issue 6(2017)
• Page Start: 1242
• Page End: 1255
• Publication Date: 2017-11-02
• Subjects: Molecularly imprinted polymer -- biomimetic immunoassay -- quantum dots -- capillary electrophoresis -- trichlorfon; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2017.1333579
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: In this study, a molecularly imprinted polymer that could selectively recognize trichlorfon was synthesized. Using the imprinted polymer as biomimetic antibody, a novel biomimetic immunoassay-capillary electrophoresis method with improved sensitivity was developed for the detection of trichlorfon. This method was based on the competitive reaction between quantum dot-labeled trichlorfon and free trichlorfon with the biomimetic antibody. Under optimal conditions, the limit of detection (IC15 ) and the sensitivity (IC50 ) of the method was 0.35 ± 0.021 μg/L and 0.81 ± 0.025 mg/L, respectively. To evaluate the accuracy of the method, trichlorfon spiked in the carrot and zucchini samples was determined, and the recovery was in the range of 80.1–95.3%. This method was also applied to the quantitative detection of trichlorfon residues in the edible rape and leek samples, and the results had a good correlation with those obtained using a gas chromatography method.