Antrodia cinnamomea mycelial fermentation broth inhibits the epithelial-mesenchymal transition of human esophageal adenocarcinoma cancer cells. (September 2018)

• Record Type: Journal Article
• Authors: Liu, Yu-Ming; Liu, Yu-Kuo; Huang, Pin-I.; Tsai, Tung-Hu; Chen, Yu-Jen
• Is Part Of: Food and chemical toxicology. Volume 119(2018)
• Page Start: 380
• Page End: 386
• Publication Date: 2018-09
• Subjects: Antrodia camphorate -- E-cadherin -- BE3 cells -- Twist -- EMT; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.01.028
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Esophageal cancer is associated with a high mortality rate and easy metastasis. The aim of this study is to investigate the effect of the bio-product Antrodia cinnamomea mycelial fermentation broth ( AC -MFB) on the epithelial mesenchymal transition (EMT) of human esophageal cancer cells and the molecular mechanisms underlying these effects. Transforming growth factor β (TGF-β) was used to induce EMT in human esophageal BE3 cancer cells. Changes in cell morphology and migration potential were examined. The expression of E-cadherin, N-cadherin, vimentin, and other transcriptional factors was studied by western blot assay. The results showed that AC -MFB was not only able to upregulate the expression of Ecadherin and attenuate the TGF-β-induced overexpression of vimentin and N-cadherin, but it also reversed the TGF-β-induced changes in cell morphology from polygonal to spindle-shaped and delayed the migration potential of BE3 cells. Furthermore, AC -MFB treatment was able to inhibit the expression levels of both Twist and Twist1. Overall, AC -MFB was able to inhibit the EMT of esophageal cancer BE3 cells, which was accompanied by Twist and Twist1 downregulation. Highlights: Esophageal cancer is associated with characters of EMT as easily metastasis and high mortality rates. AC -MFB, a biotechnologically product, from Antrodia cinnamomea . AC -MFB treatment could attenuate cellular migration, inhibit the TGF-β induce overexpression of Vimentin; reverse the E-cadherin to N-cadherin switch in esophageal cancer BE3 cells. The pathways of AC -MFB inhibit the EMT of BE3 cancer cells might be through Twist and Twist1 downregulation.