Essential oil of Citrus lumia Risso: Phytochemical profile, antioxidant properties and activity on the central nervous system. (September 2018)

• Record Type: Journal Article
• Authors: Smeriglio, Antonella; Alloisio, Susanna; Raimondo, Francesco Maria; Denaro, Marcella; Xiao, Jianbo; Cornara, Laura; Trombetta, Domenico
• Is Part Of: Food and chemical toxicology. Volume 119(2018)
• Page Start: 407
• Page End: 416
• Publication Date: 2018-09
• Subjects: Citrus lumia Risso -- Essential oil -- Antioxidant properties -- Anti-cholinesterase activity -- Cytotoxicity -- Neuroactive effects; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.12.053
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: The use of essential oils (EOs) is known since long time in traditional medicine and aromatherapy for the management of various oxidative stress-related disorders and has been further increased recently for their neuroprotective and anti-aging potentials as well as for reducing anxiety and stress. The purpose of this work was to evaluate, for the first time, the chemical composition of Citrus lumia Risso EO and its antioxidant, anti-cholinesterase, and neuroactive properties by cell-free and cell-based assays. The EO has shown strong antioxidant and free radical scavenging properties, particularly in hydrogen atom transfer based assays (β-carotene bleaching and ORAC, IC50 22 μg/mL and 46 μg/mL, respectively), that can be attributed to the high content of monoterpenes, especiallyd -Limonene (48.905%), and Linalool (18.245%). Furthermore, the EO has shown an interesting anti-acetylcholinesterase activity (IC50 258.25 μg/mL). Data from MTT analysis indicate that the cytotoxicity of EO, evaluated on L929 mouse fibroblasts, is very low, with an IC50 higher than 500 μg/mL at 48 h. Rat neuronal networks subjected to EO showed a concentration-dependent inhibition of spontaneous electrical activity. Results indicate that C. lumia EO could be an important source of natural antioxidants suggesting an important preventive role in the onset of oxidative stress-related pathologies. Graphical abstract: Highlights: Citrus lumia Risso is an ancient citrus species never investigated before. The EO showed antioxidant, anti-cholinesterase and neuroactive properties. d -Limonene and Linalool are the major constituents of lumia EO.