PBA2, a novel inhibitor of imatinib-resistant BCR-ABL T315I mutation in chronic myeloid leukemia. Issue 2 (28th December 2016)

• Record Type: Journal Article
• Authors: Gupta, Pranav; Kathawala, Rishil J.; Wei, Liuya; Wang, Fang; Wang, XiaoKun; Druker, Brian J.; Fu, Li-Wu; Chen, Zhe-Sheng
• Is Part Of: Cancer letters. Volume 383:Issue 2(2016)
• Page Start: 220
• Page End: 229
• Publication Date: 2016-12-28
• Subjects: PBA2 -- Chronic myeloid leukemia -- BCR-ABL -- T315I -- Resistance; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.09.025
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Chronic Myeloid Leukemia (CML) is largely caused by the Philadelphia (Ph) chromosome carrying the Break point Cluster Region-Abelson (BCR-ABL) oncogene. Imatinib is a BCR-ABL-targeted therapy and considered the standard of care in CML management. Resistance to imatinib therapy often develops because of mutations in the BCR-ABL kinase domain. In this study, we evaluated PBA2, a novel BCR-ABL inhibitor, for its anti-cancer activity against BCR-ABL expressing BaF3 cells. PBA2 shows potent activity against wild-type and T315I mutated BaF3 cells as compared with imatinib. PBA2 inhibited the phosphorylation of BCR-ABL and its downstream signaling in BaF3/WT and BaF3/T315I cells. PBA2 inhibited the mRNA expression of BCR-ABL in BaF3/WT and BaF3/T315I cells. Mechanistically, PBA2 increased the cell population in sub G1 phase of the cell cycle, induced apoptosis and elevated ROS production in both BaF3/WT and BaF3/T315I cells. Taken together, our results indicate that PBA2 exhibits anti-proliferative effects and inhibits the imatinib-resistant T315I BCR-ABL mutation. PBA2 may be a novel drug candidate for overcoming the resistance to imatinib in CML patients. Highlights: PBA2 is a modern inhibitor of the imatinib-resistant BCR-ABL T315I mutation. PBA2 potently inhibits the BCR-ABL signaling pathway and mRNA expression of BCR-ABL. PBA2 induces sub G1 phase and early apoptosis with subsequent increase in ROS production. PBA2 is a suitable drug candidate for the treatment against the T315I mutation in CML.