Postnatal exposure to a glyphosate-based herbicide modifies mammary gland growth and development in Wistar male rats. (August 2018)

• Record Type: Journal Article
• Authors: Altamirano, Gabriela A.; Delconte, Melisa B.; Gomez, Ayelen L.; Ingaramo, Paola I.; Bosquiazzo, Verónica L.; Luque, Enrique H.; Muñoz-de-Toro, Mónica; Kass, Laura
• Is Part Of: Food and chemical toxicology. Volume 118(2018)
• Page Start: 111
• Page End: 118
• Publication Date: 2018-08
• Subjects: Male rats -- Mammary gland -- Mast cells -- Glyphosate-based herbicide -- Estrogen receptor; AR androgen receptor -- GBH glyphosate-based herbicide -- ESR1 estrogen receptor alpha -- E2 estradiol -- IOD integrated optical density -- LN lymph node -- P&H picrosirius-hematoxylin -- PND postnatal day -- TEBs terminal end buds -- T testosterone; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.05.011
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Our aim was to evaluate whether postnatal exposure to a glyphosate-based herbicide (GBH) modifies mammary gland development in pre- and post-pubertal male rats. From postnatal day 1 (PND1) to PND7, male rats were injected subcutaneously every 48 h with either saline solution (vehicle) or 2 mg GBH/kg·bw. On PND21 and PND60, mammary gland and blood samples were collected. Estradiol (E2) and testosterone (T) serum levels, mammary gland histology, collagen fiber organization, mast cell infiltration, proliferation index, and estrogen (ESR1) and androgen receptor (AR) expression levels were evaluated. At PND21, GBH-exposed male rats exhibited greater development of the mammary gland with increased stromal collagen organization and terminal end buds (TEBs) compared to control rats. At PND60, the number of TEBs remained high and was accompanied by an increase in mast cell infiltration, proliferation and ESR1 expression in GBH-exposed male rats. In contrast, no effects were observed in E2 and T serum levels and AR expression in both days studied. Our results showed that a postnatal subacute treatment with GBH induces endocrine-disrupting effects in the male mammary gland in vivo, altering its normal development. Highlights: Postnatal subacute exposure to GBH accelerates mammary gland development in male rats. GBH augments mast cell infiltration in the mammary stroma of post-pubertal males. GBH increases mammary gland proliferation and estrogen receptor alpha expression in post-pubertal males.