An HPLC–MS/MS method for the simultaneous determination of luteolin and its major metabolites in rat plasma and its application to a pharmacokinetic study. Issue 20 (26th August 2018)

• Record Type: Journal Article
• Authors: Shi, Fuguo; Pan, Hong; Lu, Yuanfu; Ding, Li
• Is Part Of: Journal of separation science. Volume 41:Issue 20(2018)
• Page Start: 3830
• Page End: 3839
• Publication Date: 2018-08-26
• Subjects: luteolin -- mass spectrometry -- metabolite -- pharmacokinetics; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201800585
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A selective and accurate HPLC‐MS/MS method was established to simultaneously quantify luteolin and its active metabolites (diosmetin, chryseriol, and luteolin‐7‐O‐glucuronide) in rat plasma. The analytes were separated on a C18 column with a mobile phase of water containing 0.5% formic acid and acetonitrile under gradient elution to shorten the total chromatographic run time and increase the resolution of diosmetin and chryseriol. A triple quadruple mass spectrometer coupled with an electrospray ionization source in the negative ion mode was used to detect the analytes. The multiple reaction monitoring transitions were of m/z 284.9→132.9 for luteolin, m/z 298.9→283.9 for diosmetin and chryseriol, m/z 461.1→284.9 for luteolin‐7‐O‐glucuronide, and m/z 300.9→150.9 for the internal standard. The method was linear within the concentration ranges of 0.06–90 μg/mL for luteolin, 0.03–12 μg/mL for diosmetin, 0.015–4.8 μg/mL for chryseriol, and 0.06–60 μg/mL for luteolin‐7‐O‐glucuronide. The intra‐ and interday precisions were all within 6.0%. Accuracy ranged from −3.2 to 6.4%. The matrix effect and instability were not observed during bioanalysis. This method was used to study the pharmacokinetic characteristics of luteolin and its metabolites in rats after treatment with luteolin.