Expression of FcRn receptor in placental tissue and its relationship with IgG levels in term and preterm newborns. (10th May 2018)

• Record Type: Journal Article
• Authors: Lozano, Natalia A.; Lozano, Alejandro; Marini, Vanina; Saranz, Ricardo J.; Blumberg, Richard S.; Baker, Kristi; Agresta, Maria F.; Ponzio, Marina F.
• Is Part Of: American journal of reproductive immunology. Volume 80:Number 3(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-05-10
• Subjects: FcRn -- IgG -- neonates -- placenta -- preterm; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12972
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract : Problem: IgG is the only antibody class, that is, actively transferred from the mother to the fetus across the placenta by an active, neonatal Fc receptor (FcRn) mediated process during pregnancy, conferring passive immunity and protection against infections to the newborn during the first months of life. Preterm infants may not receive sufficient titers of protective antibodies, as most of them are transferred only after the 34th week of gestation. Because of the great importance of this process, we investigated in a clinical setting the placental transmission of IgG antibodies in term and preterm newborns. Method of study: This work was conducted in 85 woman and their newborns, divided into four groups according to their clinical gestational age (≤37 weeks were considered as preterm). Blood samples were collected from the mothers and their newborns' umbilical cords to analyze total serum IgG concentrations, and a subgroup of 32 placentas was analyzed by immunohistochemistry to quantify the expression of the FcRn receptor. Results: Total IgG levels in both mothers and neonates increased significantly through the third trimester of gestation. Regarding the newborns, in all groups, IgG levels exceeded their mother's values by a ~2.4%. A higher expression of FcRn was detected in placentas from newborns at week 36 of gestation onwards. Conclusion: Our results obtained from clinical samples, were in line with previous descriptions in model systems and confirmed that the IgG transfer from maternal serum to the fetus is positively correlated with FcRn expression in placental tissue throughout gestation. Abstract : Representative figure of the FcRn expression in human placental tissue (20x magnification) in term and pre‐term newborns before and after ImageJ processing (A vs. B and C vs. D, respectively).